NR AWDQ
AU Costa,C.; Vidal,E.; Tortosa,R.; Espinosa,J.C.; Torres,J.M.; Pumarola,M.
TI Water channel study (Aquaporin 1 and 4) in brains of a murine BSE model and BSE affected cattle
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PA-11
PT Konferenz-Poster
AB Aquaporins 1 and 4 belong to a family of membrane water channels involved in the water transport across the barrier between brain and other fluid compartments (blood and CSF). In the brain, AQP1 is described in the choroid plexus epithelial cells and ependimocytes. AQP4 is the most abundant isoform in the nervous tissue and it has been located mainly in astrocytes endfeet membrane. In CJD patients and in the brains of BSE and Scrapie experimentally infected mice an increase of both isoforms has been described, assessed with western blotting (A. Rodriguez, 2005). These authors suggest a possible relationship between the aquaporin expression increase and the spongiform degeneration found in TSEs. We have immunohistochemically studied the distribution of AQP 1 (Chemicon AB3065, 1:200) and AQP 4 (Chemicon AB3594, 1:200) in brains of a murine transgenic model of BSE (BoTg110), those mice overexpress the bovine cellular prion protein and have been intracerebraly challenged with a well characterized BSE inoculum (BSE1, TSE/08/59 originated from a pool of BSE cases from VLA Weybridge, U.K.). To ascertain the reproducibility of the murine model, brains from field cases of BSE cattle diagnosed in Catalonia have been also included in this study. In this poster we describe the immunostaining of both isoforms in the brains of uninfected animals and compare it with the ones affected of BSE. This study was financed by the project EET2002-05168-C04-01 of the Spanish Ministry of Science and Technology (MCyT).
AD C. Costa, R. Tortosa: Animal Medicine and Surgery Department, Veterinary Faculty, Autonomous University of Barcelona, Bellaterra (Cerdanyola del Vallès), Spain; E. Vidal: Priocat Laboratory, CReSA, Autonomous University of Barcelona, Bellaterra (Cerdanyola del Vallès), Spain; J.C. Espinosa, J.M. Torres: Animal Health Research Center (CISA-INIA), Madrid, Spain; M. Pumarola: Animal Medicine and Surgery Department, Veterinary Faculty, Autonomous University of Barcelona, Bellaterra (Cerdanyola del Vallès), Spain and Priocat Laboratory, CReSA, Autonomous University of Barcelona, Bellaterra (Cerdanyola del Vallès), Spain
SP englisch
PO Italien