NR AWDR
AU Courageot,M.P.; Bellon,A.; Daude,N.; Paquet,S.; Nonno,R.; Di Bari,M.A.; Vella,L.J.; Sharples,R.A.; Hill,A.F.; Agrimi,U.; Laude,H.; Vilette,D.
TI A cell line infectible by prion strains from various species
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions CE-08
PT Konferenz-Poster
AB We have previously shown that expression of ovine PrP renders rabbit epithelial RK13 cells permissive to the multiplication of ovine prions (Vilette et al., 2001), providing the first circumstantial evidence that efficient prion replication can occur in cultured cells from a nonneuronal origin and that the species barrier can be crossed ex vivo through the expression of a relevant PrP. We have now significantly extended our earlier observation by showing that prions adapted to different rodent species (mouse, hamster and bank vole) can be propagated in RK13 cells expressing the relevant PrP. RK13 cells expressing mouse PrP support multiplication of Fukuoka and 139A strains of murine prions while Sc237/263K strain multiply in RK13 expressing hamster PrP. In addition, BSE prions from cattle and sheep, adapted to bank vole, were found to propagate in RK13 expressing the vole PrP. Importantly, all these strains retained strain-specific banding patterns of PrPres after multiplication in cell culture. In summary, this work has identified a new cell model for hamster and mouse prions and, remarkably, the first one for BSE-derived prions. While the basis of cell permissiveness to a given strain remains enigmatic, multiplication of a range of prions from different species in the same cell model should facilitate elucidation of the molecular basis of prion diversity.
AD M.P. Courageot, A. Bellin, N. Daude, S. Paquet, H. Laude, D. Vilette: Virologie et Immunologie Moléculaires, INRA, Jouy-en-Josas, France; R. Nonno, M.A. Di Bari, U. Agrimi: Department of Food Safety and Veterinary Public Health, Istituto Superiore di Sanità, Rome, Italy; L.J. Vella, R.A. Sharples, A.F. Hill: Department of Biochemistry and Molecular Biology, Bio21, Molecular Science and Biotechnology Institute, University of Melbourne, Victoria, Australia
SP englisch
PO Italien