NR AWED
AU Dobson,C.; Heath,N.; Cook,J.; Stumpf,R.; O'Brien,A.; Clarke,J.
TI Use of probit analysis to compare rapid TSE assays
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-12
PT Konferenz-Poster
AB Assessment of assay sensitivity should ideally be made using challenging samples which have low concentrations of the analyte of choice and/or are from animals in the very early stages of the disease. The number of TSE positive samples available for assay comparison and evaluation are limited and with the declining incidence of BSE, these samples are likely to become increasing scarce. The method most widely recommended and used, for sensitivity assessment, is the testing of serial dilutions of numerous positive samples. Serial dilutions have the benefit of producing at least one sample that is close to the assay cut-off but can lead to the production of copious data which are difficult to compare and quantify. In this study the Enfer TSE Version 2 and Version 3 assays have been assessed by the testing of 140 dilution series, the data produced have been subsequently subjected to Probit analysis. The graphs used for this analysis comprise of plots of the number of samples detected as positive at each dilution against the dilution and allow a simple means of visual comparison. Additionally, an ED50 (median effective dose) has been calculated, this is the theoretical titre at which half of the samples are detected as positive. Data and analysis are also presented where Probit analysis has been used to compare Enfer assays performed manually and by automated processor and also to compare two different types of rapid TSE assays. Probit analysis has been shown to be a simple method for comparing data from the testing of multiple serial dilutions, providing quantifiable estimates of relative sensitivity (ED50) and enabling comparison of assays with differing outputs such as colorimetric, chemiluminescent and line assays.
AD C. Dobson, N. Heath, J. Cook: Murex Biotech, Dartford, UK; R. Stumpf: Abbott Laboratories, Lake Co., USA; A.O'Brien, J. Clarke: Enfer Scientific, Naas, Ireland
SP englisch
PO Italien