NR AWEI
AU Duval,C.; Comoy,E.E.; Ligouis,M.; Ulvund,M.; Cornuejols,M.J.; Couquet,C.; Adjou,K.T.; Brugere-Picoux,J.; Laude,H.; Dernis,D.; Huart,J.J.; Deslys,J.P.
TI Development of a bank of standardidized blood samples for the evaluation of future prion diagnostic tests
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-13
PT Konferenz-Poster
AB The risk of interhuman transmission of vCJD via blood is not anymore theoretical with the three cases now attributed to blood transfusion in Great Britain. For the moment, the only protective measures are based on exclusion of donors depending on their background, and on filters for leucodepletion. Screening of blood donors, like it is systematically made for different viral diseases including HIV, would constitute a reinforcement of transfusion safety. Unfortunately, the only tests available and validated in Europe for prion diagnosis are post mortem tests for TSE in ruminants, and are only able to detect PrPres in brain or peripheral organs but not in the blood. Different tests are currently under development, each of them claiming performances very difficult to estimate in the absence of common standardized samples. Moreover, most of the evaluation trials are performed with blood samples from healthy animals spiked with positive brain, which apparently reflect poorly the properties of the abnormal PrP present in blood. Last, very limited data are generated on blood samples from animals before the onset of clinical stage, to reflect the situation of patients donating blood during the silent incubation phase. In the context of the NeuroPrion Blooddiag project, we have constituted a library of blood fraction samples from sheep and goats that were experimentally or naturally infected with scrapie versus healthy controls in different research programs. All the animals were referenced and sampled regularly, and blood was fractionated with an adapted protocol, similar to the one used for humans by the French Transfusion Center of Lille (France). Infectivity of the different collected blood fractions (plasma, platelets, red cells and buffy-coat) has been assessed by inoculation of transgenic mice overexpressing ovine PrP gene (tg338). Successful transmissions have already been obtained with several samples and bioassays are still ongoing. With complete information about the animals (genotype, source of contamination, days post-contamination, presence of clinical signs), this unique library comprises now more than 8000 aliquots from the fractions of more than 110 different blood samples. This bank will constitute a powerful tool for the evaluation of the different blood tests currently under development and which already claim for future protection of blood transfusion.
AD C. Duval: CEA/DSV/DRM/GIDTIP, Prion Research Group, Fontenay-aux-Roses, France, and Etablissement Français du Sang Nord-de-France Lille, France; Emmanuel E. Comoy (comoy@dsvidf.cea.fr), M. Ligouis, J.-P. Deslys: CEA/DSV/DRM/GIDTIP, Prion Research Group, Fontenay-aux-Roses, France; M. Ulvund: The Norwegian School of Veterinary Science Sandnes, Norway; M.-J. Cornuejols, C. Couquet: Laboratoire Départemental Vétérinaire Limoges, France; K. Adjou, J. Brugère-Picoux: Ecole Nationale Vétérinaire de Maisons-Alfort, Maisons-Alfort, France; H. Laude: INRA, Virologie Immunologie Moléculaires, Jouy-en-Josas France; D. Dernis, J.-J. Huart: Etablissement Français du Sang Nord-de-France Lille, France. E-mail: emmanuel.comoy@cea.fr
SP englisch
PO Italien