NR AWEO
AU Espinosa,J.C.; Andreoletti,O.; Castilla,J.; Herva,M.E.; Morales,M.; Alamillo,E.; Lacroux,C.; Lugan,S.; Langeveld,J.; Torres,J.M.
TI Sheep-passaged BSE analysed in BoPrP-Tg110 mice exhibits BSE strain features and accelerated disease course allowing their discrimination from scrapie
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PR-11
PT Konferenz-Poster
AB We used transgenic mice expressing bovine PrP (BoPrP-Tg110) as a model to discriminate between BSE in sheep and scrapie. The transmission in BoPrP-Tg110 mice of prions from BSE infected sheep was examined and compared to the transmission of original cattle BSE and sheep scrapie prions. Our results indicate no transmission barrier for sheep BSE prions to infect BoPrP-Tg110 mice. In contrast, all three sheep scrapie isolates tested were only transmitted to BoPrP-Tg110 mice with an evident transmission barrier according to onset of disease and PrPPres scoring. The biochemical features observed in BoPrP-Tg110 mice inoculated with sheep-passaged BSE prions were similar to those rendered by the cattle BSE prions but different to those observed for the sheep scrapie isolates. These differences persisted after a second passage in these transgenic mice demonstrating that this bioassay is a valuable tool for distinguishing between BSE and scrapie in sheep.
AD J.C. Espinosa, J. Castilla, M.E. Herva, M. Morales, E. Alamillo, J.M. Torres: Centro de Investigación en Sanidad Animal. INIA, 28130 Valdeolmos, Madrid, Spain; O. Andréoletti, C. Lacroux, S. Lugan: UMR INRA-ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France; J. Langeveld: CIDC-Lelystad, PO Box 2004, 8203 AA Lelystad, The Netherlands. E-mail: jmtorres@inia.es
SP englisch
PO Italien