NR AWEP
AU Evoniuk,J.M.; Johnson,M.L.; Larson,D.M.; Taylor,J.B.; Stoltenow,C.L.; Reynolds,L.P.; Vonnahme,K.A.; Caton,J.S.; O'Rourke,K.I.; Redmer,D.A.
TI Localization and quantification of prion protein (PrPc) expression in sheep placenta
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions CE-12
PT Konferenz-Poster
AB To increase our understanding of scrapie biology, prion protein (PrPc) expression was evaluated in near-term placentas of adolescent ewes fed at moderate (MOD; 100% of National Research Council [NRC] requirements) or low (LOW; 60% of NRC) diet intake during two periods of pregnancy (day 5090 and day 90-130)], resulting in MOD-MOD, MOD-LOW, LOW-MOD, and LOW-LOW treatments. Gravid uteri (n=50) from singleton pregnancies were collected at day 130, and placentomes were perfusion fixed for immunohistochemical localization of PrPc. In addition, PrPc genotypes were determined (codons 136 and 171) using SNP assay. Most PrPc-positive cells were in the fetal placenta (trophoblast binucleate and mononucleate cells), with few localized in the maternal placenta (caruncular epithelium). PrPc protein expression (area of positive staining, graded from 1 [least] to 4 [greatest]) was less in MOD-MOD than in any other treatment (P<0.05). Overall, PrPc in maternal caruncular tissue was less (P=0.003) in MOD-MOD or LOW-MOD ewes compared to those receiving reduced nutrition in late pregnancy (MOD-LOW or LOW-LOW). PrPc was more uniformly distributed in caruncles of MOD-MOD than in any other treatment (P<0.05) and more uniform with MOD than LOW at day 90-130 (P=0.007). PrPc in fetal placenta was less uniformly distributed (P<0.01) in MOD-LOW ewes than in any other treatment. There were no genotype effects on PrPc protein expression. Thus, expression of PrPc protein is influenced by maternal nutritional level, with greatest expression in diet-restricted ewes late in pregnancy. Further study is needed to determine the role of PrPc in placental biology and scrapie transmission via the placenta.
AD J.M. Evoniuk, M.L Johnson, D.M. Larson, C.L. Stoltenow, L.P. Reynolds, K.A. Vonnahme, J.S. Caton, D.A. Redmer: North Dakota State University, Fargo, ND, USA; J.B. Taylor: USDA, ARS, USSES, Dubois, ID, USA; K.I. O'Rourke: USDA, ARS, ADRU, Pullman, WA, USA. E-mail: dale.redmer@ndsu.edu
SP englisch
PO Italien