NR AWEV
AU Fichet,G.; Comoy,E.E.; Delauney-Dehen,C.; Challier,L.; Antloga,K.; Khammo,N.; Deslys,J.P.; McDonnell,G.
TI Decontamination procedures for prions: standardization of in vitro and in vivo models for evaluation of efficiency
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions SA-04
PT Konferenz-Poster
AB Prions are unique infectious agents which have been shown to be transmitted iatrogenically, both experimentally and clinically, through contaminated surfaces and could constitute a major public health concern with regard to the large tissue distribution of the vCJD agent in humans. There is currently no standard model to evaluate the safe decontamination of surfaces, including reusable medical devices, meat processing and pharmaceutical production surfaces. We previously evaluated the efficiency of different decontamination procedures on prioncontaminated surfaces, including reference methods (bleach, sodium hydroxyde and autoclaving) and new procedures. Some of them, including new friendly detergent formulations and vaporized hydrogen peroxide, showed high efficiency against prions and are reputed to be compatible with the different surfaces to be treated. This initial study was performed by using steel surfaces contaminated with a scrapie strain adapted to hamster (263K), largely used in decontamination because of its resistance. We extended here our studies to other surfaces (plastic, glass), showing that these procedures are also efficient on them. In parallel, similar studies were performed with a BSE-related strain, adapted to mice (6PB1). As for the previous model, disease was transmitted through contaminated wires after implantation into the brains and we established a relation between the infectivity titre, the transmission rate and the incubation period. The results obtained with BSE were similar to those observed with the 263K model reinforcing the relevance of the latter for decontamination studies. The developed in vivo scrapie method is proposed as a first standard to evaluate existing and developing prion decontamination technologies. It is clear that further investigations will continue to identify practical methods for evaluating surface prion decontamination.
AD G. Fichet, K. Antloga, N. Khammo, G. McDonnell: STERIS R&D Laboratory, CEA, Route du Panorama, 92265 Fontenay-aux-Roses, France; Emmanuel E. Comoy (comoy@dsvidf.cea.fr), C. Delaunay-Dehen, L. Challier, J.-P. Deslys: CEA/DSV/DRM/GIDTIP, Route du Panorama, 92265 Fontenay-aux-Roses, France
SP englisch
PO Italien