NR AWFF
AU Gavier-Widen,D.; Renström,L.; Nöremark,M.; Ottander,L.; Klingeborn,B.; Vulin,T.J.; Baron,T.G.M.; Stack,M.J.; Chaplin,M.J.; Langeveld,J.P.M.
TI Characterization of an atypical type-H BSE case in Sweden
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PR-13
PT Konferenz-Poster
AB Bovine spongiform encephalopathy (BSE) is considered to be caused by a single strain producing a consistent and homogenous phenotype of disease in cattle. BSE had never been detected in Sweden until March 2006, when a 12-years old mixed-Charolais cow was screened positive using an ELISA test. The cow was in late pregnancy, had been recumbent and getting up repeatedly, was finally unable to rise and was euthanized. Western blot (WB) with Bio-Rad Bovine WB at SVA confirmed the case but showed that the unglycosylated band had a higher MW than in classical BSE. Further WB analysis was conducted in parallel at the VLA, AFSSA and CIDC-Lelystad. The VLA, applying mAb 6H4 in the hybrid, the OIE-SAF, and the NaPTA WB confirmed the results of SVA and also demonstrated binding of mAb P4 (specific for WGQGGSH), which is unusual for cattle. Image analysis estimated the difference of MW of being approximately 0.75-0.9 kD. Additionally, it showed more evenly distributed relative quantities of PrPsc in the di- and mono-glycosylated bands than in classical BSE. The AFSSA achieved similar results using a core antibody, Sha31, for the discrimination of apparent molecular masses and also showed binding with mAbs 12B2. CIDCLelystad showed excellent binding of 12B2 (specific for sequence WGQGG) and higher level of PK sensitivity, in contrast to classical BSE cases. IHC on brainstem, with mAbs F89 and P4 showed a characteristic widespread fine granular synaptic type of immunostaining of the neuropil throughout the grey matter in all the sections. The trigeminal tract nucleus and the solitary tract nucleus were more densely and intensively stained, and had also coarser granules. Perineuronal staining was observed in a few neurons. Notably, intracellular and glia-associated PrPd types were not observed. In conclusion, the Swedish case shows similarities with previous descriptions of H-type of BSE
AD D. Gavier-Widén, L. Renström, M. Nöremark, L. Ottander, B. Klingeborn: SVA, Upssala, Sweden; T.J. Vulin, T.G.M. Baron: AFSSA, Lyon, France; M. Stack, M. Chaplin: VLA, Webridge, UK; J.P.M. Langeveld: CIDC-Lelystad, Dept. of Bacteriology and TSEs, Lelystad, The Netherlands. E-mail: dolores@sva.se
SP englisch
PO Italien