NR AWFM
AU Gilch,S.; Kehler,C.; Prebeck,S.; Schätzl,H.M.
TI Strain and cell-type specific response of stimulated innate immune cells to prion infection
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions CE-16
PT Konferenz-Poster
AB The role of the innate immune system in prion infection has been extensively investigated during the last few years. It has been reported that application of CpG-motif containing oligonucleotides (CpGODN), which stimulate TLR9- expressing cells of the innate immune system, inhibit the progression of prion disease in mice after peripheral infection (Sethi et al., 2002). This prolongation of incubation is probably not caused by stimulation of immune cells, but by massive alterations in the architecture of the spleen, leading to a lack of follicular dendritic cells and therefore to an impaired peripheral propagation of prions (Heikenwalder et al., 2004). We used an in vitro model to study the effects of stimulation by CpG-ODN and LPS on transient prion infection of macrophages and microglia cells with different prion strains. Infection of J774 (murine macrophages) or BV2 cells (murine microglia) led to a different response dependent on the cell type and the prion strain used for infection. Furthermore, the cell surface expression of PrPc, but not the amount of PrP mRNA, was increased in stimulated cells. Unspecific effects of CpG-ODN or LPS stimulation on prion infection were excluded by employing neuronal N2a cells. Our data indicate that stimulation of innate immune cells might support transient propagation of prions in certain cell types.
AD Institute of Virology, Prion Research Group, Technical University of Munich, Germany. E-mail: gilch@lrz.tum.de
SP englisch
PO Italien