NR AWFO
AU Giles,K.; Peretz,D.; Glidden,D.V.; Prusiner,S.B.
TI Disinfection of various prion strains by acidic sodium dodecyl sulfate
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions SA-05
PT Konferenz-Poster
AB Prions are resistant to standard disinfection and sterilization procedures, resulting in well documented cases of iatrogenic prion transmission from surgical instruments. With the increase in surgical and dental procedures in the aging population, a growing number of pre-symptomatic Creutzfeldt-Jakob disease (CJD) patients are undergoing procedures and no special precautions are being taken with the instruments before reuse. We investigated the inactivation of prions by incubation with sodium dodecyl sulfate (SDS) at various pH values. As judged by sensitivity to proteolytic digestion, PrPsc was denatured at room temperature by SDS at pH values 4.5 or 10. When various concentrations of SDS and acetic acid were tested, the duration and temperature of exposure acted synergistically to inactivate both hamster Sc237 prions and human CJD prions. Using highly sensitive bioassays in transgenic mice, we found that CJD prions were over 100,000 times more resistant to inactivation than Sc237 prions, demonstrating that inactivation procedures must be validated against the strain of interest in a specifically susceptible host. As a model for surgical instrument disinfection, the inactivation of prions bound to stainless steel wires was evaluated by direct implantation into the brains of transgenic mice. Some procedures that significantly reduced prion titers in brain homogenates had a limited effect on prions bound to the surface of stainless steel wires. We have extended our studies to BSE prions, bioassayed in transgenic mice expressing bovine PrP, and to the mouse-adapted BSE strain, 301V, bioassayed in transgenic mice overexpressing the B polymorphic allele of mouse PrP. Our findings, which have been directly validated against the prion strains in the respective susceptible hosts, form the basis for a noncorrosive system suitable for inactivating prions on stainless steel surfaces.
AD K. Giles, D. Peretz: Institute for Neurodegenerative Diseases and Department of Neurology, University of California San Francisco, San Francisco, CA 94143. USA; D.V. Glidden: Department of Biostatistics, University of California San Francisco, San Francisco, CA 94143, USA; S.B. Prusine: Institute for Neurodegenerative Diseases and Departments of Neurology, Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USA
SP englisch
PO Italien