NR AWGC
AU Griffiths,P.C.; Spiropoulos,J.; Lockey,R.; Thorne,L.; Jayasena,D.; Jenkins,R.; Plater,J.M.; Tout,A.C.; Green,R.B.; Windl,O.; Beringue,V.; Le Dur,A.; Laude,H.; Buschmann,A.; Groschup,M.H.; Hope,J.
TI Transmissibility and characterisation of atypical scrapie in mice
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PR-17
PT Konferenz-Poster
AB The identification of atypical scrapie in sheep in several European countries has raised interest in the transmissibility of these agents to sheep and across species barriers. In the UK, atypical scrapie cases were detected as a result of on-going active and passive surveillance programmes for scrapie in sheep. In contrast to classical scrapie, the current characterisation of atypical scrapie cases includes: absence of vacuolation in the obex, restricted PrPsc distribution in the obex confined to the spinal tract of the trigeminal nerve, a low BioRad TeSeE ELISA signal, an extra ~12 kDa proteinase K resistant band in Western blots, and PrP genotype associated with resistance to classical scrapie. The purpose of this collaborative study is to examine whether atypical scrapie from sheep of various PrP genotypes are transmissible to transgenic PrP and conventional mouse lines, and to characterise any transmissible disease by neuropathological and biochemical means. To this end, brain homogenates from atypical scrapie sheep were inoculated into transgenic mice (Tg338, overexpressing sheep VRQ, and TgshpXI, overexpressing sheep ARQ) and wild-type mice (C57BL/6 and VM). Preliminary results have shown that transgenic mice inoculated with several atypical scrapie inocula have developed clinical signs of disease. Western blotting characterisation of brain samples from clinically affected Tg338 mice revealed Nor98-like profiles in the atypical cases examined to date (n = 10). Pathological analysis of brain samples from clinically affected Tg338 mice inoculated with atypical scrapie (6 cases) demonstrated lesion profiles indistinguishable from Nor98 and the French discordant cases, with incubation periods similar to Nor98 in Tg338 mice at ~200 days postinoculation.
AD P.C. Griffiths, L. Thorne, D. Jayasena, R. Jenkins, J.M. Plater, A.C. Tout, O. Windl: TSE Molecular Biology Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK; J. Spiropoulos, R. Lockey, R.B. Green: Neuropathology Section, Pathology Department, Veterinary Laboratories Agency, Addlestone, Surrey KT15 3NB, UK; V. Beringue, A. Le Dur, H. Laude: Virologie Immunologie Moleculaires, Institut National de la Recherche Agronomique, 78350 Jouy-en-Josas, France; A. Buschmann, M.H. Groschup: Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany; J. Hope: Veterinary Laboratories Agency, Lasswade, Midlothian EH26 0PZ, UK
SP englisch
PO Italien