NR AWGD
AU Grman,I.; Rybar,P.; Thompson,M.; Hianik,T.
TI Detection of aptamer-protein interactions using thickness shear mode technique
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-21
PT Konferenz-Poster
AB DNA or RNA aptamers can selectively bind proteins or even low molecular compounds. The selection of the method of detection of aptamer-protein interaction is important in reaching sufficient detection limit as well as for understanding the mechanisms of the protein-nucleic acid interactions. In this work we applied thickness shear mode method (TSM) for detection the interaction between DNA aptamers that selectively bind the thrombin either in heparin or fibrinogen binding sites. We also examined how the binding of thrombin depends on the aptamer structure. We showed, that binding of thrombin to the aptamer immobilized on a surface of the quartz crystal through neutravidin-biotin method is accompanied by decrease of series resonant frequency and by increase of the motional resistance. This suggests that the binding event is accompanied not only by increase of the mass of the sensing layer, but also by increasing of its shearing viscosity. The structure of aptamer significantly affected the sensitivity of the binding. Aptamer of longer oligonucleotide chain providing stabilization of the 3D structure resulted in stronger binding of thrombin in comparison with shorter aptamer without stabilization of the structure. The results obtained can be used in further application of the aptamers as a biosensors for detection e.g. prions.
AD I. Grman, P. Rybar, T. Hianik: Department of Nuclear Physics and Biophysics, FMFI UK, Mlynska dolina F1, 842 48 Bratislava, Slovakia; M. Thompson: Department of Chemistry, University of Toronto, 80 St. George Street, Toronto, Ontario M5S 3H6 Canada. E-mail: hianik@fmph.uniba.sk
SP englisch
PO Italien