NR AWGN
AU Hayward,G.L.; Stiver,W.H.; Catford,A.; Balachandran,A.V.
TI Acoustic prion sensor
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-23
PT Konferenz-Poster
AB A thickness shear mode acoustic sensor has been successfully demonstrated as a diagnostic tool to detect infectious prions. The sensor used was a standard AT cut, piezoelectric quartz crystal with gold electrodes. Commercially available, normal recombinant prion protein was immobilized on the gold surface using a thiol linking molecule. The sensor was then contacted by an homogenized brain sample and the resonant frequency of the crystal was recorded at 10 second intervals with the aid of a phase lock oscillator and computer. Scrapie infected brain and normal brain control samples from 21 sheep were tested in a total of 45 trials. No sample pretreatment beyond homogenization was required and the result was available 2 hours after the sample application. Two samples gave false positive results but no false negatives were observed. The test is based on the infection process where a normal recombinant prion protein immobilized on the sensor surface is converted to the misfolded form by an infected sample applied to the sensor.
AD G.L. Hayward, W.H. Stiver, A. Catford: School of Engineering, University of Guelph, Guelph, Canada; A. Balachandran: Canadian Food Inspection Agency, Ottawa, Ontario, Canada. E-mail: ghayward@uoguelph.ca
SP englisch
PO Italien