NR AWGO
AU Heath,N.; Dobson,C.; Cook,J.; Stumpf,R.; O'Brien,A.; Clarke,J.
TI Labour, space and resource implications of rapid assay automation
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-24
PT Konferenz-Poster
AB The Enfer TSE Version 3 assay is a 'new' and improved, qualitative immunoassay for the detection of PrPsc in central nervous tissue of cattle and small ruminants. The automation of sample homogenisation and pipetting that have previously been developed for the Enfer TSE Version 2.0 assay, are directly transferable to the Version 3 assay. Automation of the ELISA stage of the version 3 assay has now also been achieved with the en4lisa microplate processing instrument. Equipment comparisons for both the automated and manual methods of the Enfer TSE Version 3 assay have been made, demonstrating a reduction in laboratory space required for TSE testing for the en4lisa automated method. Direct comparisons of labour requirements and laboratory throughput with the Enfer TSE Version 3 manual and automated en4lisa methods, show reductions in resource requirements and increases in maximum output of laboratory areas
AD N. Heath, C. Dobson, J. Cook: Murex Biotech, Dartford, UK; R. Stumpf: Abbott Laboratories, Lake Co., US; A. O'Brien, J. Clarke: Enfer Scientific, Naas, Ireland
SP englisch
PO Italien