NR AWGY
AU Herva,M.E.; Espinosa,J.C.; Alamillo,E.; Andreoletti,O.; Torres,J.M.
TI Discrimination between scrapie and BSE in sheep using porcine PrP-transgenic mice
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PR-19
PT Konferenz-Poster
AB Previous work from our laboratory showed the existence of a strong transmission barrier for BSE prion in transgenic mice expressing porcine PrP (PoPrP-Tg). Here we study the transmission to PoPrP-Tg mice of prions from BSE infected sheep in comparison to the transmission of sheep scrapie prions. PoPrP-Tg mice were challenged with BSE infected sheep inoculum and with four different sheep scrapie isolates, in order to assess their susceptibility to the different inoculated prions. Intracerebral inoculations were performed with 10% brain homogenates. The results showed that scrapie isolates are not transmitted at all to PoPrP-Tg mice up to day (in some of them second passage is concluded). In contrast, 100% of the animals inoculated with sheep BSE inoculum succumbed to the primo-infection at approximately 450 days after inoculation. In conclusion, these data indicate a very strong transmission barrier in Po-PrP-Tg mice for sheep scrapie prions but very low for sheep BSE prions. On the other hand, these data reveal that this transgenic mouse model constitutes a valuable tool for the discrimination of BSE and scrapie in sheep.
AD M.E. Herva, J.C. Espinosa, E. Alamillo, J.M. Torres: Centro de Investigación en Sanidad Animal, INIA, Valdeolmos, Madrid, Spain; O. Andréoletti: UMR INRA-ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France. E-mail: jmtorres@inia.es
SP englisch
PO Italien