NR AWHE
AU Howlin,R.P.; Khammo,N.; Lipscomb,I.; McDonnell,G.; Keevil,C.W.
TI Investigating the efficiency of validated washer-disinfector cycles on the removal of prions from surgical stainless steel surfaces
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions SA-07
PT Konferenz-Poster
AB A key feature of the prion agent involved in the initiation and propagation of transmissible spongiform encephalopathies (TSE's) is its ability to withstand chemical and enzymatic degradation. This poses a serious concern for health services with respect to the decontamination of surgical instruments and the removal of prions: these have been shown to be transmissible via neurosurgery and contaminated neurosurgical instruments such as EEG depth electrodes. Although several methods of prion decontamination have been shown to be successful, due to their aggressive nature, none are applicable to surgical instruments. Furthermore, a validated cleaning cycle which can guarantee their complete removal from surgical instruments does not currently exist. This study simulated the conditions of validated cycles and cleaning products currently used within sterile service departments: a HAMOTM 100 prion inactivating detergent cycle with cleaning time of 15 minutes and the newly recommended 7 minutes, as well as Enzycare(R)II and Instru-Klenz(R) cleaning cycles, on surgical grade 316L stainless steel wires inoculated with a 10% mouse brain homogenate infected with ME7 Scrapie. For each cycle the pre-treatment was altered using either a Klenzyme(R) soak, a PRE-Klenz(R) spray or no treatment, as was the length of drying time of the wires following inoculation. The degree of prion removal was assessed, in addition to general organic contamination, in terms of percentage area coverage of the wires using a rapid episcopic differential interference contrast microscopy technique and a novel and sensitive fluorescent staining protocol. The efficiency of prion removal varied, depending on the pre-treatment and agents used for each cleaning cycle, as well as the effect of prior tissue drying time. The results have been shown to correlate with on going in vivo studies.
AD R.P. Howlin, I. Lipscomb, C.W. Keevil: School of Biological Sciences, University of Southampton, Southampton, SO16 7PX, UK; N. Khammo, G. McDonnell: STERIS Ltd, Basingstoke, RG22 4AX, UK. E-mail: rph@soton.ac.uk
SP englisch
PO Italien