NR AWHK
AU Iulini,B.; Perazzini,A.Z.; Porcario,C.; Corona,C.; Florio,C.L.; Langeveld,J.M.P.; Capucci,L.; Ligios,C.; Marconi,P.; Caramelli,M.; Casalone,C.
TI Atypical scrapie in Italy: immunhistochemical study with a panel of different antibodies
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PR-20
PT Konferenz-Poster
AB Fifteen cases of scrapie obtained by active surveillance with unusual features have been diagnosed in Italy since 2005. Immunohistochemical staining with our statutory antibody F99/97.6.1 showed a diffuse granular PrPsc pattern in the nucleus of the spinal tract of the trigeminal nerve and in the granular and molecular layer of the cerebellum. The purpose of this work was to investigate PrPsc deposits by immunohistochemistry using a panel of 10 monoclonal antibodies recognizing different linear epitopes dispatched among PrP sequence from the N-terminal to the C-terminal part of the protein. This study was performed in 9 animals for which the whole brain was available and different brain regions were studied including telencenphalon, diencephalons, mesencephalon, pons, cerebellum and obex. Results of this study provide comparisons between the different PrPsc deposit patterns detectable according to the epitope specificity of the antibody applied. Immunohistochemical epitope mapping of PrPsc accumulation in the brain is useful for the characterisation of atypical TSE strains in the small ruminants population.
AD B. Iulini, A.Z. Perazzini, C. Porcario, C. Corona, C.L. Florio, M. Caramelli, C. Casalone: CEA, Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta, Torino, Italy; J.M.P. Langeveld: CEA, Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta, Torino, Italy; L. Capucci: Istituto Zooprofilattico Sperimentale della Lombardia e dell' Emilia Romagna, Brescia, Italy; C. Ligios: Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy; P. Marconi: Istituto Zooprofilattico Sperimentale delle Regioni Lazio e Toscana, Firenze, Italy
SP englisch
PO Italien