NR AWHL
AU Iwamaru,Y.; Hoshino,M.; Takenouchi,T.; Ogihara,K.; Takata,M.; Imamura,M.; Yoshiba,S.; Masujin,K.; Ushiki,Y.K.; Okada,H.; Kitani,H.; Yokoyama,T.
TI Various mouse-adapted prion strains infection of a novel microglial cell line
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions CE-19
PT Konferenz-Poster
AB Prion diseases are progressive, degenerative diseases of the nervous system. The histological characteristics of prion diseases are spongiform change of brain, neuronal loss, astrocytosis and increase of activated microglia. The relevance of microglia in the pathogenesis of prion diseases has been inferred by many studies, but the precise roles of microglia still remain largely obscure. To elucidate the roles of microglia in the pathogenesis of prion diseases, physiological and biochemical characterization of prion-infected microglial cells should be clarified. In this work, we report a novel prion-infected microglial cell culture model. After exposure to Chandler scrapie, a microglial cell line (MG20 cell) established from the brain of neonatal tga20 mice that overexpress murine prion protein, replicated disease-associated forms of the prion protein as well as infectivity. In addition, Chandler scrapie maintained biological properties in this cell culture. Furthermore, MG20 cells were susceptible to various prion strains, such as ME7, Obihiro scrapie and bovine spongiform encephalopathy agents. Thus, this cell line may provide a potentially valuable tool for the investigation of the molecular events in microglia that participate in the pathogenesis of prion diseases and prion strain determinants.
AD Y. Iwamaru, M. Hoshino, M. Takata, M. Imamura, S. Yoshiba, K. Masujin, H. Okada, T. Yokoyama: National Institute of Animal Health, Tsukuba, Japan; T. Takenouchi, K. Ogihara, H. Kitani: National Institute of Agrobiological Sciences, Tsukuba, Japan; Y.K. Ushiki: Nippi Research Institute of Biomatrix, Tokyo, Japan. E-mail: gan@affrc.go.jp
SP englisch
PO Italien