NR AWHM
AU Jacobs,J.G.; Langeveld,J.P.M.; Biacabe,A.G.; Acutis,P.L.; Mazza,M.; Polak,M.; Gavier-Widen,D.; Buschmann,A.; Groschup,M.H.; van Zijderveld,F.G.; Davidse,A.; Baron,T.G.M.
TI TSEs in cattle: discrimination of three molecular types of protease resistant prion protein in brain diagnosed with bovine spongiform encephalopathy
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PR-21
PT Konferenz-Poster
AB Transmissible spongiform encephalopathies (TSEs) exhibit strain specific properties by their behaviour in bioassays and in molecular analyses of the disease associated prion protein (PrPsc). However, bovine spongiform encephalopathy (BSE) of cattle appeared to be very homogeneous. Recently, a limited number of atypical isolates have been identified in several countries. This asks for a comparative study to find a firm molecular classification of these cases. Various conditions of digestion with proteinase K (PK) and PNGaseF, analysis by Western blot with a range of epitope-defined antibodies were explored. Analyses of the disease associated proteinase K resistant moiety of PrP (PrPres), as described previously by Biacabe and Casalone, confirmed existence of two additional PrPres variants, defined here as type-H and type-L following the higher and lower position of the aglycosylated PrPres band in Western blots compared to that in BSE. This was further confirmed by varying the biochemical conditions like detergent addition, PK digestion, and deglycosylation treatment. The three molecular PrPres types exhibited different migration patterns, glycoprofile, PKsusceptibilities and antibody binding. These properties appear independent of geographical origin suggesting the general occurrence of these two different PrPres variants in cattle, possibly all over the world. A practical procedure for the recognition of these types in brain tissues has been worked out.
AD J. Jacobs, J.P.M. Langeveld, F.G. von Zijderveld, A. Davidse: CIDC-Lelystad, Dept. Bacteriology & TSEs, Lelystad, The Netherlands; A.G. Biacabe, T. Baron: AFSSA, Lyon, France; P.L. Acutis, M. Mazza: CEA - Istituto Zooprofilattico del Piemonte, Turin, Italy, Turino, Italy; M. Polak: NVRI, Dept. Virology, Pulawy, Poland; D. Gavier-Widén: SVA, Upssala, Sweden; A. Buschmann, M.H. Groschup: FLI-INEID, Riems, Greifswald, Germany
SP englisch
PO Italien