NR AWHP
AU Johnson,M.L.; Luther,J.S.; Wallace,J.M.; Evoniuk,J.M.; Reynolds,L.P.; Stoltenow,C.L.; O'Rourke,K.I.; Redmer,D.A.
TI Effects of nutrition and genotype on cellular prion protein (PrPc) mRNA expression in ovine placental tissues
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions CE-20
PT Konferenz-Poster
AB To determine the effects of nutrition and genotype on PrPc mRNA expression, singleton pregnancies from a single sire were established by embryo transfer to adolescent dams, which then were offered a moderate (M, n=22), high (H, n=20), or high-low (HL, with high to low switch at day (d)90 of gestation, n=10) diet intake. Whole placentomes, collected at d90 or 130 of gestation, were separated into maternal, (caruncle, CAR) and fetal (cotyledon, COT) tissues for quantitative real-time RT-PCR determination of PrPc mRNA expression. PrPc genotypes were determined for codons 136 and 171 using SNP assay. In CAR, across all treatments, PrPc mRNA was greater (P<0.001) on d90 than on d130, but in COT it was similar on both days. On d90, PrPc mRNA tended to be greater (P<0.08) in CAR than in COT, but on d130, PrPc mRNA was greater (P<0.001) in COT than in CAR. There were no effects of nutrition on d90 of pregnancy. However, on d130, for CAR and COT, PrPc mRNA was reduced (P<0.01) in H compared to M, but was similar in M and HL. PrPc mRNA expression was unaffected by codon 136 genotype. In CAR on d90 and in COT on d130, PrPc mRNA was greater in ewes with an R at codon 171 than in those with QQ (1.78±0.14 vs 0.98±0.25 for CAR, P<0.02; 1.64±0.10 vs 1.31±0.13 for COT, P<0.06). Thus, differences in PrPc mRNA expression in fetal and maternal placental tissues were influenced by nutrition, day of pregnancy, and genotype. Further study is needed to determine whether changes in PrPc mRNA expression affect scrapie transmission via the placenta.
AD M.L. Johnson, J.S. Luther, J.M. Evoniuk, L.P. Reynolds, C.L. Stoltenow, D.A. Redmer: Dept. of Animal and Range Sci., North Dakota State University, Fargo, ND, USA; J.M. Wallace: Rowett Research Institute, Aberdeen, Scotland, UK; K.I. O'Rourke: USDA, ARS, ADRU, Pullman, WA, USA. E-mail: mary.lynn.johnson@ndsu.edu
SP englisch
PO Italien