NR AWHY
AU Kirby,L.; Walker,J.T.; Dickinson,J.; Dennis,M.J.; Sutton,J.M.; Fulford,M.R.; Hall,G.A.; Marsh,P.D.; Perrett,D.; Smith,A.J.; Ironside,J.; Bruce,M.; Raven,N.D.H.
TI Novel models to assess the iatrogenic transmission risks for vCJD in dentistry using BSE-301V in VM mice
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions SA-08
PT Konferenz-Poster
AB The potential for transmission of vCJD by dentistry has been suggested to be very low based on current risk assessments, and there is no evidence to suggest that people have been infected through this route. To provide further underpinning for such assessments, this study will help to identify the risk of iatrogenic transmission between patients during certain invasive dental procedures. Groups of VM mice (n=10) were inoculated with BSE-301V by scoring infected orthodontic dental files along their gum line and leaving in the gingival margin tissue for 5 minutes. The 100 mice were then culled bimonthly (2 up to 20 months) to assess the speed and route of BSE-301V infection in the VM mouse, and to provide evidence for relative infectivity of different tissues. 10 tissues including brain, spleen and various oral tissues have been harvested. These tissues will then be processed and reinoculated intra-cranially into VM (n=6) mice to assess the infectivity of these tissues. To date this study has completed the primary infections and collected the range of tissues from the time course, which are now being processed for re-inoculated. This study will be complemented by assessing the infectivity of oral vCJD tissues in additional ongoing studies. These human tissues will be injected intra-cranially into RIII mice and scored after a fixed time-point. These results will be discussed with respect to our understanding of instrument decontamination and the potential transmission of (v)CJD via general surgery.
AD L. Kirby, J.T. Walker, J. Dickinson, M.J. Dennis, J.M. Sutton, M.R. Fulford, G.A. Hall, P.D. Marsh, N.D.H. Raven: TSE Research Group, Health Protection Agency, Porton Down, Salisbury, Wiltshire SP4 0JG, UK; D. Perrett: William Harvey Research Institute Barts & the London School of Medicine & Dentistry Charterhouse Square London EC1M 6BQ, UK; A.J. Smith: Infection Research Group, Glasgow Dental Hospital & School, 378 Sauchiehall Street, Glasgow G2 3JZ, UK; J. Ironside: National CJD Surveillance Unit, Edinburgh, Scotland, UK; M. Bruce: Institute for Animal Health, Edinburgh, Scotland. Email: liz.kirby:hp.org.uk
SP englisch
PO Italien