NR AWIO
AU Lai,J.; Decastelli,L.; Ru,G.; Ingravalle,F.; Monaco,A.; Abete,M.C.
TI Evaluation of the microscopic method for the detection of animal derived constituents in feedingstuffs
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions SA-09
PT Konferenz-Poster
AB The BSE (Bovine Spongiform Encephalopathy) epidemic in Europe has heighten the need to adopt strict control measures to avoid the risk of spreading of the disease through meat and bone meal (MBM) based animal feedingstuffs. The aim of this study was to evaluate the reproducibility and the accuracy of the microscopic examination (Directive 2003/126/EC), as official method to evaluate the presence of MBM and to identify the animal classes, by a ring trial. CReAA (National Reference Centre for surveillance and monitoring of animal feed) provided nine European labs with a set of 35 samples prepared using a commercial feedingstuff for calves as common matrix: positive samples were made by adding a 0.1% concentration of animal MBM meal (either mammal or chicken or fish). The reproducibility of the microscopic method was very good: k-overall was 0.83 (95%CI: 0.77 - 0.88). With regard to accuracy considering the results in terms of presence/absence of MBM the sensitivity was equal to 100% (95%CI: 95.98 - 100.00) and the specificity was 94.67% (95%CI: 90.87 - 97.21) for all the raters together. In terms of kind of constituent (eg. mammal, poultry, fish) the specificity was 97.13% (95%CI:94.43-98.75) for fish, 98.61% (95%CI:96.48-99.62) for mammal and 98.61%(95%CI:96.48-99.62) for chicken. Sensitivity showed a high variability among the animal classes: fish 100% (95%CI: 90.26-100), mammal 48.15% (95%CI:26.67-68.05 ) and chicken 44.44% (95%CI:25.48-64.67). The microscopic method show high level of accuracy and reproducibility with some limits in discriminating between chicken and mammal contamination. Acknowledgments to the other European laboratories.
AD J. Lai, L. Decastelli, A. Monaco: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Turin, Italy; G,.Ru, F. Ingravalle: CEA Centro di Referenza Nazionale per le Encefalopatie Animali Turin, Italy; M.C. Abete: C.Re.A.A. Centro di Referenza Nazionale per il monitoraggio e il controllo per l'Alimentazione Animale Turin, Italy. E-mail: controllo.alimenti@izsto.it
SP englisch
PO Italien