NR AWIQ
AU Langeveld,J.P.M.
TI Rapid and discriminatory diagnosis of TSEs in lymph nodes of CWD infected wild life animals
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre, Workshop of the Neuroprion Cervid Group - Chronic Wasting Disease (CWD): Current knowledge and European perspective 2006, 3.10.2006
PT Konferenz-Vortrag
AB
Live and early screening in deer and elk for CWD would offer a tool for the determination of the TSEs status of wild life. Using our recently published method* for detection of scrapie and BSE in retropharyngeal lymph nodes (RLN) we applied the methods on some lymph nodes from white tailed deer, mule deer and elk. Ovine scrapie and BSE infected RLNs were used for comparison. Using antibodies SAF32, 12B2, L42, and F99 at respectively 0.5, 0.2, 0.2 and 2 µg/ml in Western blots and 8 mg tissue equivalents per lane (except elk RLN, 0.4 mg TE), the method was capable to detect the positive animals. The molecular aspects of the deer and elk PrPres samples appeared to differ from those in scrapie and BSE infected sheep RLN. Also, the antibody reactivities confirm that the CWD samples can be considered different from BSE. Methods and other molecular aspects will be discussed within the limits of the experiments. The present data indicate that WB of suitable lymph nodes can be a promising and sensitive tool for applying surveillance of live wild game for CWD because of its speed and sensitivity.
*Langeveld JPM, Jacobs JG, Erkens JHF, Bossers A, van Zijderveld FG, van Keulen LJM. 2006. Rapid and discriminatory diagnosis of scrapie and BSE in retro-pharyngeal lymph nodes of sheep. BMC Veterinary Research 2:19. We thank Dr. Aru Balanchandran (National Reference Laboratory for Scrapie and CWD, Animal Diseases Research Institute, Canadian Food Inspection Agency, Ottawa, Ontario, Canada) for providing CWD-samples. This work was performed with funding of the Dutch agricultural Ministry of LNV and NeuroPrion FOOD-CT-2004-506579 project "ruminant TSE strain typing".
AD CIDC-Lelystad, Wageningen-UR, PO Box 2004, 8200AA, Lelystad, The Netherlands
SP englisch
PO Italien