NR AWJA
AU Lescoutra-Etchegaray,N.; Comoy,E.E.; Jacquin,S.; Brown,P.; Lasmezas,C.; Deslys,J.P.
TI Standardized blood samples of Creutzfeldt-Jakob-infected primates for the evaluation of future prion diagnostic tests
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-34
PT Konferenz-Poster
AB Several sensitive diagnostic tests are under development to detect pre-clinical infection with variant Creutzfeldt-Jakob disease (vCJD) in order to minimize the risk of secondary transfusion-associated infections. Their evaluation will be hindered by the impossibility of creating a library of blood samples from humans incubating vCJD. We propose a practical alternative using a non-human primate model (Macaca fascicularis) inoculated with several prion strains including vCJD. These animals are currently under study in our laboratory, and are having blood drawn on a regular basis during the pre-clinical and clinical phases of disease. We adapted fractionation procedures from blood transfusion centres to macaque blood to obtain plasma, buffy-coat and red blood cells comparable to those currently processed in humans. Samples are already available from ten primates infected with autopsy verified infections with BSE or vCJD. Fifty animals inoculated with different strains for a variety of research programmes are currently under surveillance and sampled regularly. This library of standardized blood samples, clearly identified and correlated to the clinical status of the animals, will constitute an important resource for a future validation of screening tests.
AD N. Lescoutra-Etchegaray, E. Comoy, S. Jacquin, J.-P. Deslys: CEA/DSV/DRM/GIDTIP, 18 route du Panorama, 92265 Fontenay-aux-Roses, France; P. Brown: Bethesda, Maryland 20814, USA; C. Lasmézas: CEA/DSV/DRM/GIDTIP, 18 route du Panorama, 92265 Fontenay-aux-Roses, France, and Bethesda, Maryland 20814, USA, and Department of Infectiology, Scripps Florida, 5353 Parkside Drive, RF-2 Jupiter, FL33458, USA. E-mail: nathalie.lescoutra@cea.fr
SP englisch
PO Italien