NR AWJB
AU Ligios,C.; Sigurdson,C.; Carta,A.; Carcassola,G.; Cancedda,M.G.; Madau,L.; Uras,P.; Galistu,A.; Santucciu,C.; Maestrale,C.; Crudeli,S.; Attene,S.; Aguzzi,A.
TI PrPsc deposits in lungs and mammary glands of sheep experimentally coinfected with scrapie and maedi-visna virus
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PA-32
PT Konferenz-Poster
AB Scrapie, a fatal neurodegenerative disorder of sheep, is characterized by deposition of an abnormal isoform of prion protein (PrPsc) in the central nervous system (CNS) and within the lymphoreticular system (LRS). Recent studies in mice transgenically engineered to develop organ specific inflammation demonstrated the co-occurrence of PrPsc in the inflamed organs (kidney, pancreas and liver). To test the possibility that ectopic PrPsc replication occurs in sheep affected with scrapie and chronic inflammations, Sarda breed lambs of three different PrPnp genotypes (136/154/171: ARQ/ARQ, ARQ/ARR and ARR/ARR, n=10 each) were inoculated with Maedi-Visna virus (MVV) intratracheally and intravenously, and with scrapie brain homogenate by the oral route (n= 10/group). Control lambs (n=10/group) were inoculated with scrapie brain homogenate only, or with mock brain (n=2/group). Nine of 10 ARQ/ARQ sheep inoculated with scrapie and MVV showed neurological signs, starting from 19 months post-inoculation. Eight sheep were diagnosed with scrapie by immunohistochemistry (IHC) and western blotting (WB) of brain and tonsil. Preliminary results demonstrated that 4 of the 8 scrapie affected sheep had PrPsc deposits in the inflamed mammary gland (lymphofollicular mastitis). Furthermore, 3 of these 8 sheep had a chronic progressive pneumonia and PrPsc accumulation as detected by IHC and/or WB.
AD C. Ligios, M.G. Cancedda, L. Madau, P. Uras, A. Galistu, C. Santucciu, C. Maestrale, S. Crudeli, S. Attene: Istituto Zooprofilattico Sperimentale della Sardegna, Sassari, Italy; C. Sigurdson, A. Aguzzi: UniversitätsSpital Zürich, Institute of Neuropathology Department of Pathology, Zürich, Switzerland; A. Carta: Istituto Zootecnico Caseario della Sardegna, Olmedo, Italy; G. Carcassola: Dip. di Patologia Animale, Igiene e Sanità Pubblica Veterinaria, Sez. di Microbiologia e Immunologia, Milano, Italy. E-mail: ciriaco.ligios@izs-sardegna.it
SP englisch
PO Italien