NR AWJF
AU Luhr,K.M.; Löw,P.; Taraboulos,A.; Kristenson,K.
TI Stainless steel composition as a determining factor for prion binding
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions SA-11
PT Konferenz-Poster
AB Prions are uniquely resistant to conventional sterilization procedures and inactivation by various chemical treatments. This resistance of prions to inactivation has led to several cases of prion transmission following inadequate sterilization of injected materials both in animals and humans. Prion-contaminated electrodes have been implied in spreading prion infections and, and since prions may accumulate in lymphoid tissues for long periods of times, surgical instruments used in operations on non-symptomatic prion-infected individuals are at a potential risk for transmission of diseases. This has led to the awareness that instruments and materials used in dentistry, surgery and slaughtering may be a source of prion spread. In the present study we investigate whether metals and/or stainless steel of different composition differ in their prion binding capacity. We here report that stainless steel powders of different composition, exposed for 24 hrs to scrapie (RML-strain)-infected GT1-1 cell lysates, show a marked difference in their binding of PrPsc as determined by Western blotting. We also observed that the difference in prion binding capacity of the steel powders was at least partly due to their difference in nickel content. Thus, stainless steel devoid of nickel may be considered for use in instruments that are at risk of being exposed to prions. This study was supported by grants from US Army DAMD17-03-102288 and by EC LSHBCT-2006-019090
AD K.M. Luhr, P. Löw, K. Kristensson: Department of Neuroscience, Karolinska Institutet, Stockholm, Sweden; A. Taraboulos: Dept. Mol. Biology, Hadassah Medical School, Jerusalem, Israel. E-mail: katarina.luhr@ki.se
SP englisch
PO Italien