NR AWJQ
AU Manson,J.C.; Cancellotti,E.; Tuzi,N.L.; Hart,P.; Wiseman,F.; Barron,R.M.; Bishop,M.T.; Ironside,J.; Will,R.
TI TSE strains and the role of PrP in host susceptibility
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Oral sessions ORAL-22
PT Konferenz-Vortrag
AB The full extent of the diversity of TSE strains in animals and humans has not yet been established. Distinct TSE strains have been identified in mice by serially passaging infectious material from sheep, goats, cattle and humans and multiple possible variations in strains are being identified in these species by PrP glycotyping. Extensive surveillance for BSE and scrapie in Europe and elsewhere has revealed the existence of a number of previously unrecognised TSE strains. It has not yet been established whether these represents a newly diversified strains of TSE or whether they have existed for some time and been identified through the increased surveillance. TSE strains have been shown to be associated with differences in conformation, degree of protease resistance and glycoform ratios. Using our glycosylation deficient mice we are addressing the relationship between these characteristics and the TSE strain. The ability of a TSE strain to infect a new host is thought to lie in the sequence or structural similarity between the host PrP and that of the donor of infectivity. Host PrP is clearly a major factor determining susceptibility and we have demonstrated in vivo using gene targeted transgenic mice that changes in the species of PrP and also single amino acid changes within a species can have a dramatic but often unpredictable effect on host susceptibility. Moreover we have demonstrated that alterations in host PrP glycosylation can alter susceptibility not only within a species but also between species. Understanding the mechanism underlying host susceptibility, strain determination and strain targeting remains however a major challenge for TSE research.
AD J.C. Manson, E. Cancellotti, N. Tuzi, P. Hart, F. Wiseman, R.M. Barron: Institute for Animal Health, Neuropathogenesis Unit, Ogston Building, KB, West Mains Road Edinburgh EH9 3JF UK; M.T. Bishop, J. Ironside, R. Will: National CJD Surveillance Unit, Edinburgh, UK
SP englisch
PO Italien