NR AWJW
AU Maurella,C.; D'Angelo,A.; Possidente,R.; Careddu,M.E.; Bona,M.C.; Crescio,M.I.; Ratto,L.; Tarable,F.; Cellerino,C.; Caramelli,M.; Ru,G.
TI Evaluation of a clinical protocol to detect scrapie
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions EPI-16
PT Konferenz-Poster
AB This work presents the results of a three-years study carried out in a large outbreak of scrapie affecting sheep of Biellese breed. Aims of the study were to record clinical signs consistent with scrapie, to evaluate the accuracy of a standardised protocol in detecting the disease. 49 animals were submitted to a complete neurological examination. A three-part standardized data collection form was filled in for each animal and an ad hoc database was implemented. For clinical history taking, a list of the main clinical signs found in Italian outbreaks was provided to obtain an objective record of the signs the breeder described. The neurological examination followed the standard procedure for assessing mental status, posture, gait, postural reactions and proprioception, cranial nerves, spinal reflexes and sensitivity. A positive clinical history for scrapie included at least two clinical signs as reported by the breeder. A sheep was considered suspicious if it met with at least two of the following criteria: positive clinical history, abnormal fleece, abnormal mental status/behavior, abnormal gait, abnormal postural reaction/proprioception, legs dirty with ruminal material or saliva and positive nibble reflex. The accuracy of the clinical test has been evaluated using the routinary confirmatory tests (histopathology, immunohistochemistry) as the Gold Standard. 18 animals were scrapie affected out of 49 sheep clinically examined. By using the software Stata 9.2, sensitivity, specificity. and predictive values have been calculated. On the whole this clinical test showed good diagnostic performances: the positive predictive value was 93% (CI95% 66-100) whereas the sensitivity was not so good (Se 72%; CI95% 47-90). Moreover the test showed a good ability in avoiding false positive results (Sp 97%; CI95% 83-100) and, although the high prevalence in the study group, a good negative predictive value (NPV 86% CI95% 70-95).
AD C. Maurella, R. Possidente, M.E. Careddu, M.C. Bona, M.I. Crescio, M. Caramelli, G. Ru: Istituto Zooprofilattico del Piemonte, CEA, Turin, Italy; L. Ratto, F. Tarable: ASL 18, Bra, Cuneo, Italy; C. Cellerino: Regione Piemonte, Direzione di Sanità Pubblica, Italy; A. D'Angelo: Faculty of Veterinary Medicine, Turin-Italy
SP englisch
PO Italien