NR AWKE
AU Milhavet,O.; Casanova,D.; Chevallier,N.; McKay,R.D.G.; Lehmann,S.
TI Neural stem cell model for prion propagation
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PA-39
PT Konferenz-Poster
AB Study of prion transmission and targeting is a major scientific issue with important consequences for public health. Only few cell culture systems able to convert the prion protein, PrPc, into its pathologic isoform, PrPsc, have been described. We hypothesized that CNS neural stem cells (NSCs) could be the basis of a new cell culture model permissive to prion infection. CNS neural stem cells are the self-renewing, multipotent cells that generate neurons, astrocytes, and oligodendrocytes in the nervous system. Here, we report that monolayer cultures of differentiated fetal NSCs or adult multipotent progenitor cells isolated from mice were able to propagate prions. Moreover, orientation of cell differentiation experiments allowed us to demonstrate the large influence of neural cell fate on the production of PrPsc allowing the molecular study of prion neuronal targeting in relation with strain differences. This new stem cell based model, which is applicable to different species and to transgenic mice, will allow rapid and thoughtful investigations of the molecular basis of prion diseases and will open new avenues for diagnostic and therapeutic research. This work is supported by the Neuroprion network of excellence (LOI Cellprion Test), DEFRA (SE2002), and the CNRS.
AD O. Milhavet, D. Casanova, N. Chevallier, S. Lehmann: Institut de Génétique Humaine, CNRS-UPR1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France; R.D.G. McKay: Laboratory of Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892, USA. E-mail: Ollivier.Milhavet@igh.cnrs.fr and Sylvain.Lehmann@igh.cnrs.fr
SP englisch
PO Italien