NR AWKM
AU Monleon,E.; Acin,C.; Bolea,R.; Galindo,I.; Monzon,M.; Salazar,E.; Serrano,C.; Vargas,M.A.; Badiola,J.J.
TI In vivo scrapie diagnosis by PrPsc detection in lymphoid tissue: a comparative study using third-eyelid and rectal mucosa biopsies
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-37
PT Konferenz-Poster
AB Scrapie of sheep and goat is an endemic disease in many countries. The control and eradication of this disease have been hampered by the lack of a practical ante mortem diagnostic test. It is known that PrPsc is detectable in lymphoid tissue of scrapie infected animals even before development of the clinical disease. In most of the susceptible sheep, PrPsc is firstly detectable in the Peyer's patch and its draining mesenteric lymph node; afterwards, in most of the gut-associated lymphoid tissues and in other lymphoreticular system tissues. Ante mortem diagnostic tests based on the detection of PrPsc in palatine tonsil, third-eyelid and rectal mucosa associated lymphoid tissue biopsies have been described. Although the palatine tonsil biopsy is not a practical field procedure, both third-eyelid and rectal mucosa associated lymphoid tissue biopsies have been proposed for scrapie screening analysis in the field. The objective of this study was to compare the performance of in vivo scrapie diagnostic tests using third-eyelid and rectal mucosa associated lymphoid tissue biopsies in field conditions. Animals belonging to a regularly monitored flock with a high incidence of natural scrapie and genetically susceptible sheep coming from infected flocks detected through the Spanish active surveillance program were included in this study. A sample from rectal mucosa and another one from third-eyelid lymphoid tissue were collected from each animal under local anesthesia. All of them were formaldehyde fixed and routinely processed for histological and immunohistochemical examination. The results of this field study confirmed that both biopsy samples (third -eyelid and rectal mucosa lymphoid tissue) were suitable for in vivo scrapie diagnosis. However, third-eye biopsy caused a higher degree of distress in the animals and therefore it was more difficult to immobilize them for sampling.
AD Department of Animal Pathology. University of Zaragoza. Spain. E-mail: emonleon@unizar.es
SP englisch
PO Italien