NR AWLA
AU Nocentini,M.; Abete,M.C.; Pavino,D.; Focardi,C.
TI Validation of a FT-NIR microscopy method for the screning of bone particles in the sediment fraction of feeding stuff
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions SA-12
PT Konferenz-Poster
AB
The aim of the present study is to validate as screening method the FT-NIR microscopy technique for the analysis of animal meals in feeds. A commercial feed has been spiked at three different concentration levels, 0.05%, 0.1% and 0.3%, Each sample, at the three different concentration, has been analyzed in ten replicates. The sedimentation fraction has been separated from the samples following the same preparation procedure of the official method (Directive 2003/126 EC), and then analyzed with the FT-NIR mycroscopy. The NIR spectra have been collected in the range between 6000-4000 cm -1, with a resolution of 8 cm -1, the data have been further elaborated by using the mapping technique. Following the same procedure,35 samples of the proficiency testing organized in Italy by C.Re.A.A in 2005, have been tested, and the results obtained compared with those of participants using the official method. The following parameters ha been defined:
- Specificity, selectivity
- Stability/ruggedness
- Detection limit
- Precision
It has also been demostrated that the false compliant rate is <0.5% (ß error), as it is demanded in the case of screening method. A linear correlation has been found between the results obtained at the three different concentration levels. This method should be used in the laboratory for feed control as screening method; in the case of a suspected non-compliant result, this should be confirmed by the official method.
AD M. Nocentini, C. Focardi: Istituto Zooprofilattico Sperimentale Lazio e Toscana Florence Italy; M.C. Abete, D. Pavino: Centro di Referenza Nazionale per la Sorveglianza ed il Controllo degli Alimenti per Animali (C.Re.A.A) Torino Italy. E-mail: mila.nocentini@izslt.it
SP englisch
PO Italien