NR AWLI
AU Oelschlegel,A.M.; Geissen,M.; Groschup,M.H.
TI Infection studies to determine the susceptibility of cell lines to different TSE-strains
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions CE-37
PT Konferenz-Poster
AB Cell culture models are an essential part of the prion research and may facilitate a better understanding of the cellular and molecular processes leading to the formation and accumulation of the disease marker protein (PrPres) and to the infectious agent itself. Cell lines, susceptible to prions and replicating PrPres, provide a basis for these studies. Unfortunately, only a limited number of these cell lines exist until now and the susceptibility of these is strictly limited to certain, mainly mouse adapted prion strains. In our study, we examined a variety of cell lines from different eukaryotic species, tissues and developmental stages for their susceptibility to several prion strains. Moreover we generate a number of transgenic cell lines, expressing PrPc of heterologous species under the control of different expression systems and investigated, whether this expression leads to an increased susceptibility. We analysed to what extend a successful infection of cell lines is determined by culture conditions or infection protocols and several detection methods were compared to verify even low levels of PrPres accumulations. Infected cell lines were analysed and characterized in regard to strain- and species barriers, the feasibility to increase the PrPres production by selection as well as to the stability of the infection under different conditions. A large number of cell lines could not be infected or could be infected and lost PrPres after a very short period of time. Nevertheless, we were successful in infecting some of the transgenic cell lines with RML and one not transgenic bovine cell line with natural sheep scrapie. In the majority of cases the susceptibility to prions was limited to a certain species, but not necessarily to certain strains. Cell lines successfully infected with TSE were stable, the infection was repeatable and the accumulation of PrPres could be increased by selection.
AD Institute for Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Isle of Riems, Germany
SP englisch
PO Italien