NR AWLP
AU Parnham,D.; Drummond,D.; Halliday,S.I.; Foster,J.D.; Murphy,L.; Gossner,A.; Houston,E.F.; Hopkins,J.; Hunter,N.
TI Pathogenesis of SSBP/1 scrapie in sheep of susceptible and resistant PrP genotypes
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PA-41
PT Konferenz-Poster
AB SSBP/1 is a source of experimental scrapie that causes clinical disease following subcutaneous inoculation in sheep of PrP genotypes encoding at least one VRQ allele. VRQ/VRQ animals have shorter incubation periods than the heterozygotes VRQ/ARR by around 100 days whereas animals of ARR/ARR genotype resist the infection and do not develop clinical disease. In this project a time course study was carried out for the first time to follow spread of SSBP/1 infection in the peripheral lymphoreticular system and at different sites in the brain of the sheep using PrPSC as a marker, detected by immunohistochemistry using two different anti-PrP antibodies BG4 (N terminal epitope) and R145 (C terminal epitope). The time course starts at 10 days after infection and continues to terminal stages in the susceptible sheep. Differences were found in the patterns of disease-related PrP deposition between VRQ/VRQ and VRQ/ARR sheep and between the patterns detected by the two antibodies. The resistant ARR/ARR sheep were also been examined to detect any low levels of PrPSC which would be indicative of subclinical infection. The results will be presented and the implications for diagnostic tests discussed.
AD D. Parnham, D. Drummond, J.D. Foster, N. Hunter: Neuropathogenesis Unit, Institute for Animal Health, West Mains Road, Edinburgh, EH9 3JF, UK; Sue I. Halliday (sue.halliday@bbsrc.ac.uk), E. Fiona Houston (fiona.houston@bbsrc.ac.uk), Institute for Animal Health, Compton Laboratory, Compton, Newbury, Berkshire RG20 7NN, UK; L. Murphy: Division of Animal Production and Public Health, Faculty of Veterinary Medicine, Glasgow University, Bearsden Road Glasgow G61 1QH, UK; A. Gossner, J. Hopkins: Veterinary Biomedical Sciences, R(D)SVS, University of Edinburgh, Summerhall, Edinburgh EH9 1QH, UK. E-mail: nora.hunter@bbsrc.ac.uk
SP englisch
PO Italien