NR AWLS
AU Pedersen,J.A.; Ma,X.; Benson,C.H.; McKenzie,D.; Aiken,J.M.
TI Pathogenic prion protein adsorption to quartz sand
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions EPI-21
PT Konferenz-Poster
AB Soil may serve as a reservoir for prion infectivity, and soil-bound prions remain infectious. The mechanisms controlling the environmental transport of prions and their interaction with soil particles remain poorly understood. As an initial step toward understanding prion interaction with soil, we conducted batch sorption experiments to examine PrPsc adsorption to quartz sand as a function of pH, ionic strength and prion protein concentration. The electrokinetic properties of both PrPsc and the quartz sand were determined under relevant solution conditions, as was PrPsc aggregate size. Prion protein interaction with quartz surfaces was strongly pH-dependent with maximal sorption occurring near the apparent isoelectric point of the PrPsc aggregates. Our findings were in qualitative agreement with predictions based on colloid stability theory. Our results suggest that the composition of the soil solution influences prion mobility in soils and therefore the accessibility of soil-associated prions to grazing animals and other species that ingest soil.
AD J.A. Pedersen, X. Ma: Department of Soil Science, University of Wisconsin, Madison, WI, USA; J. Pedersen, X. Ma, H. Benson: Department of Civil and Environmental Engineering, University of Wisconsin, Madison, WI, USA; J.A. Pedersen, D. McKenzie, J.M. Aiken: Department of Animal Health and Biomedical Sciences, University of Wisconsin, Madison, WI, USA. E-mail: joelpedersen@wisc.edu
SP englisch
PO Italien