NR AWMA
AU Plews,M.; Simon,S.L.R.; Boreham,D.R.; Mitchel,R.E.; Czub,S.; Knox,J.D.
TI The role of oxidative stress in prion provoked neurotoxicity
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PA-43
PT Konferenz-Poster
AB There is a wealth of evidence implicating oxidative stress in the pathology of many neurodegenerative diseases. In prion diseases the accumulation of PrPsc correlates with disease progression and coincides with the appearance of markers of oxidative stress in the brains of infected mice. This suggests that the disease-associated neurodegeneration may in part be due to oxidative damage. To investigate the role of oxidative stress in prion provoked neurotoxicity the influence of a dietary supplement on oxidative stress and disease progression was determined. Brains and body fluids were collected at various time points throughout the course of the disease and markers of antioxidant capacity, DNA damage and lipid peroxidation were measured. Scrapie infected mice displayed reduced total glutathione levels as compared to control mice. The appearance of decreased total glutathione levels relative to control was delayed in infected mice fed the anti-oxidant diet. Later stages of the disease were marked by an increase in 8-OHdG levels. The anti-oxidant diet caused a reduction in 8-OHdG levels, but they remained elevated relative to that observed in age-matched controls. Infection did not alter 4-HNE levels. The data demonstrates that oxidative stress is associated with prion disease well before clinical signs are apparent. Though the anti-oxidant diet may have ameliorated adverse effects resulting from the disease-associated oxidative stress the onset of terminal stage disease was not delayed.
AD M. Plews, J.D. Knox: Department of Medical Microbiology, Faculty of Medicine, University of Manitoba, Winnipeg, Canada, and Division of Host Genetics and Prion Diseases, Public Health Agency of Canada, Winnipeg, Canada; S.L.R. Simon: Division of Host Genetics and Prion Diseases, Public Health Agency of Canada, Winnipeg, Canada; D.R. Boreham: Department of Medical Physics and Applied Radiation Sciences, McMaster University, Hamilton, Ontario, Canada; R.E. Mitchel: Radiation Biology and Health Physics Branch, Atomic Energy of Canada Limited, Chalk River Laboratories, Stn 51, Chalk River, Canada; S. Czub: National BSE Reference Laboratory, National Centre for Foreign Animal Disease/Canadian Food Inspection Agency, Winnipeg, Canada. E-mail: Margot_Plews@phac-aspc.gc.ca
SP englisch
PO Italien