NR AWMF
AU Porcario,C.; Ferraris,M.M.; Casalone,C.; Iulini,B.; Perazzini,A.Z.; Pira,E.; Manea,B.; Herrero Hernandez,E.; Bozzetta,E.; Caramelli,M.; Valentini,C.
TI Identification of metals accumulation in brains of healthy and scrapie affected sheeps by magnetic resonance imaging
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-42
PT Konferenz-Poster
AB Magnetic resonance imaging (MRI) is widely used to investigate metals accumulation in brain. The aim of this study is to set up a method to carry out MRI on sheep brain, avoiding artefacts due to interface tissue-air, and to investigate metals presence in healthy and scrapie-affected brains to detect possible differences. MRI was performed on four healthy brains preserved and prepared with different protocols. Optimal images, due to absence of morphological artefacts and signal alteration, were obtained dipping whole brains in a plastic container containing 10% formaldehyde solution. To obtain a good quality of imaging several sequences (SE, FSE, GE, and FLAIR) have been performed and images were acquired in the sagittal, coronal and axial planes. Subsequently, we analyzed four brain of healthy and scrapie-affected sheep, carrying out MRI in double blind. Correlation between hyper-intensity and a high abnormal Manganese accumulation, taking into account a possible influence of formalin fixation on Mn concentration in cortex is under investigation. Imaging showed hyper-intensity in cortex (surface layer) and pineal region for every examined sample; pattern of hyper-intensity is consistent with Manganese accumulation, due to the paramagnetic nature of this element. Use of MRI to discriminate between toxic and physiological concentration of Mn will be evaluated.
AD C. Porcario, C. Casalone, B. Iulini, A.Z. Perazzini, B. Manea, E. Bozzetta, M. Caramelli: CEA Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta, Torino Italy; M.M. Ferraris, C. Valentini: Unità Operativa di Neuroradiologia, CTO Torino, Italy; E. Pira, E. Herrero Hernandez: Traumatology, Orthopaedics and Occupational Health Department, University of Turin, Italy. E-mail: cea.neuropatologia.ufficio@izsto.it
SP englisch
PO Italien