NR AWMG
AU Pregel,P.; Corona,C.; Bollo,E.; Martucci,F.; Mazza,M.; Gazzuola,P.; Gallo,M.; Biolatti,B.; Laude,H.; Poli,G.; Casalone,C.; Caramelli,M.
TI Methodological approaches to cell culture models of TSE: slowing of cell growth increases prion replication
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions CE-40
PT Konferenz-Poster
AB The ability of prions to replicate in vitro is restricted to a few cell lines. Cultures exposed to prions produce only low levels of infectious prions, apparently because only a small percentage of cells become infected. A limiting factor in obtaining a successful infection of cells could be the high rate of cell division, which doesn't allow the prion enough time to replicate itself. In the present investigation, N2a58 (a mouse neuroblastoma cell line overexpressing mouse PrP), Rov (a rabbit kidney cell line expressing the ovine VRQ allele of PrP in a doxycycline-dependent manner) and Mov cells (immortalized neuroglial cells isolated from mice expressing ovine PrP) were cultivated in soft agar in order to slow their growth and allow a more efficient prion infection. Different culture media were tested. Growth curves showed a longer period for cell proliferation. This finding has potential implications in terms of designing new cell systems more permissive to prion replication in vitro.
AD P.Pregel, E. Bollo, B. Biolatti: Università degli Studi di Torino, Dipartimento di Patologia Animale, V. L. Da Vinci 44, 10095 Grugliasco (TO), Italy; C. Corona, F. Martucci, M. Mazza, P. Gazzuola, M. Gallo, C. Casalone, M. Caramelli: CEA-Istituto Zooprofilattico Sperimentale del Piemonte Liguria e Valle d'Aosta, Via Bologna 148, 10154 Turin, Italy; H. Laude: Unité de Virologie Immunologie Moléculaires INRA 78350 Jouy-en-Josas, France; G. Poli: Università degli Studi di Milano - Facoltà di Medicina Veterinaria, Milan Italy. E-mail: paola.pregel@unito.it
SP englisch
PO Italien