NR AWNN
AU Serrano,C.; Harders,F.L.; Lyahyai,J.; Monleon,E.; Bolea,R.; Badiola,J.J.; Zaragoza,P.; Martin-Burriel,I.; Bossers,A.
TI Identification of gene expression changes in naturally scrapie infected sheep SNC using a sheep CDNA microarray
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PA-50
PT Konferenz-Poster
AB The pathogenesis of Scrapie and many neurodegenerative diseases is poorly understood. The identification of genes with differential expression in CNS of infected animals might provide clues to clarify the molecular mechanism that leads to neuronal loss, being useful for future therapies and to identify molecular biomarkers that might be the basis for new diagnostic tests. We present here an initial study on the transcriptional differences of cerebellum obtained from naturally infected Scrapie sheep using cDNA microarray hybridizations. We have used the sheep cDNA microarray generated in the CIDC of Lelystad (see the communication of Bossers et al. for details). Total RNA of cerebellum was isolated from 5 control sheep and 9 infected sheep. According to IHC characteristics on cerebellum (CER), spleen (SP) and mesentery lymph node (MN) of infected sheep their RNAs were grouped into 4 pools (1: +CER, +SP, +MN; 2: -CER, -SP, -MN; 3: +CER, -/+SP, -MN; 4: -CER, +SP, +MN). The remaining (5) pool was formed with the 5 controls. The five RNA pools were hybridized against a universal reference RNA, after cDNA synthesis and fluorescent labeling. We compared "in silico" gene expression of the 4 positive groups against the control group. One common clone was identified to be up-regulated within the 4 diseased groups and two clones identified as down-regulated. In the two groups with PrP deposit on cerebellum (1 and 2) we found 2 upexpressed clones and 4 down-expressed clones in common. The other two groups (2 and 4) shared 6 clones with significative expression increase and 3 clones with decreased expression. We also obtained other clones with significative differential expression compared with controls. To confirm these results we are analysing the expression of selected clones by Real Time PCR.
AD C. Serrano, J. Lyahyai, P. Zaragoza, I. Martín-Burriel: Laboratorio de Genética Bioquímica, Universidad de Zaragoza, Spain; F.L. Harders, A. Bossers: Central Institute for Animal Disease Control (CIDC-Lelystad), The Netherlands; E. Monleón, R. Bolea, J. Badiola: Centro Nacional de Referencia para las EETs, Universidad de Zaragoza, Spain. E-mail: cserrano@unizar.es
SP englisch
PO Italien