NR AWOF
AU Spraker,T.R.; Powers,J.G.; Watry,M.K.; Wild,M.A.
TI Lymphoid involvement in pre-clinical chronic wasting disease in adult mule deer (Odocoilus hemionus)
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-48
PT Konferenz-Poster
AB The exact dissemination mechanism of the chronic wasting disease (CWD) associated prion, PRPcwd, within natural cervid hosts is not completely understood. Strong evidence suggests that the peripheral nervous system is important in this process. In addition, the tropism of PRPcwd for lymphoid tissue has been extensively described. PRPcwd has been demonstrated in various lymphoid tissues of experimentally and naturally infected mule deer (Odocoileus hemionus). However, the extent to which PRPcwd is distributed in lymphoid tissues of pre-clinical free-ranging mule deer has not been reported. We describe six, apparently healthy, male and female mule deer, sampled as part of an ongoing test and cull CWD management program at Rocky Mountain National Park, Colorado, USA. Each deer was found to have immunohistochemical staining (IHC) consistent with CWD in a palatine tonsil biopsy sample. Deer were euthanized and full necropsies performed. Numerous tissues were collected to investigate the distribution of PRPcwd during the pre-clinical stages of disease. Five of the animals were considered to be in the early stages of CWD and one was in a mid disease stage. Lymphoid tissues of the head, neck, thorax, and abdomen including peripheral lymph nodes were IHC positive. Additionally, hemal nodes of the neck, thorax and abdomen were positive. The thymus glands were IHC negative. This report documents the wide distribution of lymphoid tissues where PRPcwd may be found during the early stages of CWD in mule deer. Furthermore, PRPcwd accumulation in hemal nodes suggests the potential for hematogenous dissemination of PRPcwd.
AD T.R. Spraker: College of Veterinary Medicine and Biomedical Sciences, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, 80526 USA; J.G. Powers, M.A. Wild: Biological Resources Management Division, National Park Service, Fort Collins, CO, 80525, USA; M.K. Watry: Rocky Mountain National Park, Estes Park, CO, 80517, USA
SP englisch
PO Italien