NR AWOG
AU Spraker,T.R.; Gidlewski,T.; Balachandran,A.V.; VerCauteren,K.C.; Creekmore,L.; Munger,R.D.
TI Utility of rectal mucosal biopsies for detecting chronic wasting disease in Rocky Mountain elk (Cervus elaphus nelson)
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-49
PT Konferenz-Poster
AB Preclinical diagnostic tests for transmissible spongiform encephalopathies have been described for mule deer (Odocoileus hemionus), using biopsy tissues of palatine tonsil; for sheep, using lymphoid tissues from palatine tonsil, third eyelid and rectal mucosa. The utility of rectal mucosal biopsies was investigated in Rocky Mountain elk (Cervus elaphus nelsoni), a species for which there is not a live-animal diagnostic test. Postmortem rectal mucosal sections (PRMS) were examined from 308 elk from two herds that were depopulated. The results of the PRMS were compared to immunohistochemical (IHC) staining of the brain stem (vagus nucleus), retropharyngeal lymph nodes and palatine tonsil. Seven elk were found positive in the brain stem and cranial lymphoid tissues, however, only six of these elk were positive in the PRMS. The remaining 301 elk in which PRPcwd was not detected in the brain stem and cranial lymphoid tissues also were free of PRPcwd in the PRMS. Rectal mucosal biopsies (RMB) were taken from 421 live elk from three ranches in which at least two previous cases of CWD had occurred. Three positive non-clinical elk were found in one farm. These elk were killed and CWD was confirmed. PRPcwd also was found around neurons and in non-myelinated nerves within the submucosa. PRPcwd also was found in the connective tissue of the lamina propria of the infected elk. Number of follicles counted in PRMS and biopsies decreased with age of animal. This procedure is easy and rapid to perform and may be suitable as a diagnostic test as part of an integrated management strategy to detect CWD in elk.
AD T.R. Spraker: College of Veterinary Medicine and Biomedical Sciences, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, Colorado, 80526 USA; T. Gidlewski, L. Creekmore: USDA/APHIS, Veterinary Services, Fort Collins, CO 80521, USA; A. Balachandcran: Animal Diseases Research Institute, Canadian Food Inspection Agency, Nepean, Ontario, Canada; K.C. VerCauteren: National Wildlife Research Center, USDA/APHIS, Wildlife Services, Fort Collins, CO, 80521, USA; R.D. Munger: USDA/APHIS, Veterinary Services, Lincoln, Nebraska, USA
SP englisch
PO Italien