NR AWON
AU Strammiello,R.; Cescatti,M.; Farquhar,C.; Marshall,A.; Mai,J.; Beekes,M.; Parchi,P.
TI Physico-chemical properties of PrPres in peripheral tissues of experimental TSEs
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PR-31
PT Konferenz-Poster
AB The discovery of strain-related PrP-res physico-chemical properties has provided a potentially new approach for rapid strain typing in TSEs, but the lack of knowledge about PrP-res characteristics in peripheral tissues represents a significant obstacle. We studied PrP-res properties in brain and some peripheral tissues in: i) 20 mice orally exposed to the ME7 scrapie strain at different times after exposure, ii) 2 hamsters intracerebrally (ic) infected with ME7, 2 hamsters orally infected with 263K at terminal stage, and iv) 15 mice ic infected with 11 distinct scrapie and BSE strains, also at terminal stage. Because of the relatively low PrP-res content and the presence of non-specific signals, we used extraction procedures such as NaPTA precipitation and purification in Sarkosyl rather than crude homogenate analyses. The electrophoretic mobility of PrP-res showed a consistent pattern in all the TSE models analysed, unaffected by the type of tissue or stage of disease. As the only exception the 301C and 301V BSE strains in mice were distinguishable because of a faster gel migration. As shown in previous studies, PrP-res glycotype was highly heterogenous among different TSE strains and a quite distinctive feature in some of them. PrP-res glycotype was largely maintained in peripheral tissues in all the models analysed; as the only exception the hamsters i.c. inoculated with the ME7 strain showed a tissue-specific glycotype in the gut. The amount of PrP-res in peripheral tissues significantly differed among the TSE models analysed and never reached the amount detected in the brain. Our preliminary results indicate that, despite the limitations related to the low amount of PrP-res accumulation and the presence of a tissue-specific effect in some cases, biochemical strain typing can be also fruitfully applied to PrP-res extracted from peripheral tissues. Supported by EU contract QLG3-CT-2002-81030.
AD R. Strammiello, M. Cescatti, P. Parchi: Department of Neurological Sciences, University of Bologna, Bologna, Italy; C. Farquhar, A. Marshall: Institute for Animal Health, Edimburgh, UK; J. Mai, M. Beekes: Robert Koch Institute, Berlin, Germany. E-mail: a parchi@neuro.unibo.it
SP englisch
PO Italien