NR AWOQ
AU Tang,Y.; Xiang,W.; Mountjoy,S.; Saunders,G.; Kretzschmar,H.A.; Windl,O.
TI Gene expression profiles of scrapie infected mouse brain cells
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions GEN-27
PT Konferenz-Poster
AB The aims of this study were to understand the overall effect of prion pathogenesis on gene expression and to identify differentially regulated genes in scrapie infected mouse brain (SMB) cells. These cell lines are commonly employed in prion research and can be maintained as infected and uninfected forms. We analyzed the expression profiles of SMB cells using Affymetrix GeneChip Mouse Genome 430 2.0 Arrays, which contain 45,101 probe sets. The data were normalized and analyzed using the GeneSpring software. Clustering analysis showed that the results were highly reproducible between biological repeats. 8,618 genes passed the filter showing 2 fold or greater change in expression, while only 313 genes passed the filter of 8 fold change. Among these 313 genes several genes were found to have a link with prion diseases in previous studies, for example: complement component 2, complement component factor h, histocompatibility 2, matrix metalloproteinase 2 and neuropeptide Y. Many of these 313 genes encode proteins involved in immune response, apoptosis, cell adhesion, stress response and transcription. To validate the micoarray data, TaqMan quantitative PCR of six genes were carried out and the results showed even greater differences between the scrapie infected samples and the controls in gene expression. These data provide insights into the cellular responses during scrapie infection and also offer a model for expression profiling experiments in prion diseased animals.
AD Y. Tang, S. Mountjoy, G. Saunders, O. Windl: TMB deparment, Veterinary Laboratories Agency, New Haw, UK; W. Xiang, H.A. Kretzschmar: Center for Neuropathology and Prion Research, Ludwig-Maximilians University, Munich, Germany
SP englisch
PO Italien