NR AWOS
AU Tayebi,M.; David,M.; Morton,R.; Pollard,J.; Hawke,S.
TI Specific anti-prion antibodies lead to neuronal apoptosis
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions THE-18
PT Konferenz-Poster
AB The mechanism(s) of the prion-mediated neuronal degeneration are not fully understood. Previous studies have shown that neurones that lack PrPc expression are unable to sustain accumulation and deposition of PrPsc. Furthermore, lack of PrPc fails to trigger any neurotoxic effects that are normally seen with PrPsc, indicating that PrPsc is not the only factor responsible for neuropathological changes to take place. PrPc may also play an important role in the control of neuronal survival as has been demonstrated by complexation of PrPc to anti-prion antibodies that led to neuronal apoptosis. Our report shows that treatment of neuronal cells with anti-prion antibodies led to a dramatic overexpression of cytosol apolipoprotein E (apoE), release of cytochrome c from mitochondrial compartment and ultrastructural changes consistent with apoptosis. Our current data demonstrate that fine selection of antibodies to be used in prion therapeutic context is possible and even be encouraged although caution should be exerted when considering prion-mediated immunotherapy approaches.
AD Department of Medicine/Neurology, Central Clinical School The University of Sydney, Blackburn Building D06 NSW 2006 Australia
SP englisch
PO Italien