NR AWOW
AU Thorne,J.K.; Everest,D.J.; Wilson,S.; Sauer,M.J.
TI Time resolved fluoroimmunoassay of PrPsc in sheep blood
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-52
PT Konferenz-Poster
AB Development of a pre-symptomatic blood test for TSEs for human and veterinary diagnostic purposes remains a high priority. This also represents an appreciable challenge given the low levels of the disease biomarker PrPsc likely to be present in blood. One of the few tests reported as capable of detecting PrPsc in blood is capillary electrophoresis immunoassay (1) (ICE), however this procedure appears to lack the robustness required for routine application. A microwell based time resolved fluoroimmunoassay (trFIA), analogous with ICE, has been developed and applied to the analysis of blood following extraction with a solid phase 'PrPsc specific' affinity ligands. These ligands, known as the "Seprion range" are a group of polymeric compounds that have the ability to bind PrPsc, under selective conditions. Plasma and buffy coat Seprion captured extracts from 30 sheep at the terminal stage of scrapie and 30 control sheep were analysed using the novel trFIA. Highly significant differences were seen between the diseased and control animal groups, for both the plasma and buffy coat samples (p = <0.0001). This study provides preliminary proof of principle for the combined Seprion extraction -trFIA procedure, which offers promise as a pre-mortem diagnostic blood test for scrapie. 1. Schmerr, M.J., et al. (1999) J. Chromatog. A, 853, 207-214. Funding: Food Standards Agency, project MO3035
AD J.K. Thorne, D.J. Everest, M.J. Sauer: Veterinary Laboratories Agency, New Haw, Addlestone, Surrey, KT15 3NB, UK; S. Wilson: Royal College Street, London, NW1 0NH, UK
SP englisch
PO Italien