NR AWOX
AU Thorne,L.; Barnicle,D.A.; Jenkins,R.; Everest,S.J.; Gough,K.C.; Owen,J.P.; Whitelam,G.C.; Kilpatrick,J.; Jackman,R.; Terry,L.A.
TI Differentiation of animal TSE strain types using proteinase K digestion patterns of disease-associated PrP
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions PR-35
PT Konferenz-Poster
AB Variation in the stability of the disease-associated prion protein (PrPd) associated with transmissible spongiform encephalopathies to enzymic proteolysis has recently been used to help identify different strains of sheep scrapie. The aim of this work was to determine if differences in PrPd proteolysis patterns could be used to identify cases of BSE in sheep. Using proteinase K digestion profiles obtained from SDS PAGE western immunoblot (number, size and intensity of protein bands at increasing digestion times), we compared the PrPd from brain homogenates of sheep experimentally infected with BSE and naturally incurred scrapie in sheep. Resistance to proteinase K (PK) digestion was measured by the binding of epitope specific antibodies to sample proteins extracted after prolonged digestion in modified immunoassays. Significant differences in susceptibility to PK digestion were observed between strains and host and banding patterns in western blots identified molecular characteristics of the digestion products. Similar methods are being employed to characterise scrapie field isolates. Funding: defra UK
AD L. Thorne, D. Barnicle, R. Jenkins, S.J. Everest, R. Jackman, L.A. Terry: Veterinary Laboratories Agency Weybridge, New Haw, Addlestone, Surrey, KT15 3NB, UK; K.C. Gough, J.P. Owen, J. Kilpatrick: ADAS Rosemaund, Preston Wynne, Hereford, HR1 3PG, UK; G.C. Whitelam: Department of Biology, University of Leicester, Leicester, LE1 7RH, UK. E-mail: s.everest@vla.defra.gsi.gov.uk
SP englisch
PO Italien
EA Poster, Übersicht 1, Übersicht 2, Ausschnitt 1, Ausschnitt 2