NR AWOY
AU Tiers,L.; Peoc'h,K.; Roche,S.; Laplanche,J.L.; Lehmann,S.
TI In search for blood TSE biomarkers using the SELDI (surface-enhanced laser desorption/ionization) approach
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-53
PT Konferenz-Poster
AB The present work relies on the use of the new SELDI (Surface-Enhanced Laser Desorption/Ionization) proteomic approach to look for TSE related biomarkers. Importantly, the analysis of biological samples and in particular serum is analytically challenging due to the high dynamic concentration range of constituent protein/peptide species. However, SELDI has already demonstrated its ability in proteomic sample analysis leading to the discovery and development of novel multimarker clinical assays for different cancers as well as for neurological disorders. In the framework of a Neuroprion program (STEM-TSE), we are analyzing different TSE samples including human, ovine, mouse and cattle sera. We will present here the SELDI approach and give examples of the results obtained using human sera. We have indeed selected different peaks which relative level of expression were linked to the presence of a human TSE and/or to a neurodegenerative process. We are now in the process of purifying and identifying the biomarker candidates. This work is supported by the Neuroprion network of excellence (LOI SELDI-TSE) and the CNRS.
AD L. Tiers, S. Roche: Institut de Génétique Humaine, CNRS-UPR1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France; K. Peoc'h, J.L. Laplanche: Service de Biochimie et Biologie Moléculaire, Hôpital Lariboisière, 2 rue A. Paré, 75475 Paris cedex 10, France; S. Lehmann: Institut de Génétique Humaine, CNRS-UPR1142, 141 rue de la Cardonille, 34396 Montpellier Cedex 5, France, and Service de Biochimie et Biologie Moléculaire, Hôpital Lariboisière, 2 rue A. Paré, 75475 Paris cedex 10, France. E-mail: Sylvain.Lehmann@igh.cnrs.fr
SP englisch
PO Italien