NR AWPC
AU Torres,J.M.; Espinosa,J.C.; Andreoletti,O.; Herva,M.E.; Alamillo,E.; Lacroux,C.
TI BSE agent shows an enhanced virulence after passage in sheep
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Oral sessions ORAL-18
PT Konferenz-Vortrag
AB Sheep are fully susceptible to BSE infection and the ensuing disease is un-differentiable from scrapie in terms of pathogenesis and clinical signs. The biological properties of BSE adapted in sheep, and more particularly its capacity to infect other species is an animal and human health concern. We investigated, using transmission in various mice models, biological properties of BSE passaged in sheep by comparison to the original cattle BSE and various sheep scrapie prions. Our results indicate, in all mice models we used (bovine, porcine, ovine or murine), that sheep BSE have an increased attack rate and/or a reduced incubation time when compared to the effects of the original cattle BSE isolate. The reduced incubation duration was conserved in subsequent passage in BoPrPTg mice indicating that alterations of biological properties in BSE in sheep are not due to differences in infectious titre. In the meanwhile, pathological and biochemical features in mice inoculated with sheep BSE prions were very similar to those exhibited by cattle BSE prions but differed from those observed with sheep scrapie isolates. The enhanced virulence of BSE after passage in sheep raises new concerns about the infectivity of this new prion to infect other species including human.
AD J.M. Torres, J.C. Espinosa, M.E. Herva, E. Alamillo: Centro de Investigación en Sanidad Animal. INIA, 28130 Valdeolmos, Madrid, Spain; O. Andréoletti, C. Lacroux: UMR INRA-ENVT 1225, Interactions Hôte Agent Pathogène, Ecole Nationale Vétérinaire de Toulouse, Toulouse, France. E-mail: jmtorres@inia.es
SP englisch
PO Italien