NR AWPF
AU Tsenkova,R.N.; Sakudo,A.; Tei,K.; Nakao,A.; Ikuta,K.
TI In-vivo diagnosis of prion infected mouse using visible and infrared light
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions DIA-56
PT Konferenz-Poster
AB To be truly useful, prion diagnostics should identify "suspect" cases during the pathogenesis at as early stage as possible. The currently available methods are quite insensitive when compared with those available for other infectious diseases. Near Infrared Spectroscopy (NIRS) has proved to be a straight forward method for disease diagnosis based on noninvasive and nondestructive in vivo monitoring of bio fluids and tissue spectral changes. We have examined possible application of NIRS for prion diagnosis. Scrapie (Chandler and Obihiro strains) infected C57BL/6J mouse brain homogenate was used as the challenge material. Twenty microl of 10% brain homogenates were suspended in 1ml of PBS and intracerebrally injected into the brain of 20 and 4 mice, respectively. Brain homogenate of a normal mouse and PBS, as controls, were injected in 4 mice each. NIR in-vivo spectra of each mouse were acquired in the area of the abdomen and the brain, respectively, at regular time intervals, with portable NIR instrument, Fruit tester 20 (Fantec Ltd., Japan). Spectral data were analyzed by Principal component analysis (PCA) and SIMCA classificator and the difference between groups was expressed as distance between classes. The experiment was carried out until the number of the mice in infected groups became 1 (226 days, 20 spectral measurements at regular time intervals). It was found that the distance between each class of infected mice and the control group (mice with normal brain homogenate injected) was significant and increasing with the time while the distance between the control and the PBS injected mice was decreasing. Interestingly, periodic ups and downs of the class distances to the control were observed for all groups, over the period of the experiment. In this experiment, the first substantial distance was found at day 50, for Chandler, and at day 90, for Obihiro strain, after the injection. NIRS proved to be a valuable tool for an early in-vivo diagnosis and for bio - monitoring that could provide new scientific insides in the area of prion disease diagnosis and understanding of its pathogenesis.
AD R. Tsenkova, K. Tei, A. Nakao: Graduate School of Science and Technology, Kobe University, Kobe, Japan; A. Sakudo, K. Ikuta: Department of Virology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan. E-mail: rtsen@kobe-u.ac.jp
SP englisch
PO Italien