NR AWQD
AU Walker,J.T.; Dickinson,J.; Kirby,L.; Budge,C.; Dennis,M.J.; Hesp,R.; Sutton,J.M.; Fulford,M.R.; Hall,G.A.; Marsh,P.D.; Perrett,D.; Smith,A.J.; Raven,N.D.H.
TI Transmission of BSE-310V following infection from the small intestine; a new model for investigating iatrogenic transmission risks for vCJD
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions S-28
PT Konferenz-Poster
AB The study explores the use of a novel challenge model designed to provide information on the levels of infectivity of tissues following infection from the small intestine. The model uses direct challenge via the small intestine to prevent the contamination of the oral cavity by the primary inoculum. Groups of VM mice (n=10) were inoculated with 100ul of 2% w/v BSE-301v infected brain homogenate directly into the small intestine and sacrificed at 3, 6, 9, 12, 15, 18, 21 weeks post-inoculation. Tissues including spleen, saliva, salivary gland, trigeminal ganglia, gingival margin, alveolar bone, dental pulp, posterior tongue, anterior tongue, brain were removed at the appropriate time points and reinoculated (intra-cranially) into groups of mice (n=6) which were sacrificed at a clinical end point. Results to date indicate that brain and spleen from mice inoculated via this route became infectious very early in the course of disease progression and achieved maximum infectious titres well before clinical symptoms became apparent. Western blot analysis for the presence of PrPsc in the brain samples does not correlate well with the levels of infectivity and this is currently being investigated further. These results will be discussed in connection with our understanding of the iatrogenic transmission risks for (v)CJD via general surgery, endoscopy and dental practice.
AD J.T. Walker, J. Dickinson, L. Kirby, C. Budge, M.J. Dennis, R. Hesp, J.M. Sutton, G.A. Hall, P.D. Marsh, N.D.H. Raven: TSE Research Group, Health Protection Agency, Porton Down, Salisbury, Wiltshire SP4 0JG, UK; M.R. Fulford: c/o Health Protection Agency, Porton Down, Salisbury, Wiltshire SP4 0JG, UK; D. Perett: William Harvey Research Institute, Barts & the London School of Medicine & Dentistry, Charterhouse Square, London EC1M 6BQ, UK; A.J. Smith: Infection Research Group, Glasgow Dental Hospital & School, 378 Sauchiehall Street, Glasgow G2 3JZ, UK. E-mail: jimmy.walker@hpa.org.uk
SP englisch
PO Italien