NR AWQQ
AU Witusik,M.; Hulas-Bigoszewska,K.; Krynska,B.; Azii,S.A.; Liberski,P.P.; Rieske,P.
TI The role of PrPc protein in neural differentiation of NSCs into neuronal and glial line
QU International Conference - Prion 2006: Strategies, advances and trends towards protection of society - 3.10.-6.10.2006, Torino, Italy, Lingotto Conference Centre - Poster sessions CE-54
PT Konferenz-Poster
AB The definite role of PrPc protein has not been established yet, however one of PrPc putative roles is its involvement in cell differentiation. The aim of this study was to investigate the possible role of PrPc in differentiation of neural stem cells (NSCs) into neuronal and glial cells, induced in vitro by serum deprivation. We examined the expression pattern of PRNP gene in relation to differentiation at mRNA (RT-PCR) and protein level (immunocytochemistry). We detected the expression of PRNP gene in all steps of differentiation, however, no significant changes in PrP mRNA level were observed. Immunocytochemical analysis demonstrated the presence of PrPc protein during all steps of neuronal cells differentiation which were marked by expression of stage specific proteins: nestin, ß-III-tubulin, MAP-2. The expression of PrPc was also detectable in subset of GFAP-positive maturating glial cells. Our results suggest that PrPc expression during differentiation is not restricted to the neuronal cells only, since we revealed PrPc expression in glial cells as well. It may imply PrPc involvement in differentiation of astrocytic and neuronal cells. (The study was partially supported by KBN grant No. 2P05A 13 29)
AD M. Witusik, K. Huilas-Bigoszewska, P.P. Liberski, P. Rieske: Department of Molecular Pathology and Neuropathology, Chair of Oncology, Medical University of Lodz, 8/10 Czechoslowacka str., 92-216 Lodz, Poland; B. Krynska, S.A. Azizi: Department of Neurology, Temple University School of Medicine, 3401 N Broad St. 558 Parkinson Pavilion, Philadelphia, PA 19140, USA
SP englisch
PO Italien